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BDU Biophysics and Instrumentation Sem-II
No Units Titles Sub Titles Chapters
1 Unit 1 NA Scope and methods in Biophysics, Levels of molecular organization, Thermodynamics, Atomic structure of molecules - Conformational analysis and forces, Physical properties of Biomolecules, Molecular interaction of Biopolymers, Biophotonic and its application, Structure of ion channels - Biophysical, Biochemical aspects of ion channels, Voltage depended membrane permeability VIEW CHAPTERS
2 Unit 2 NA Introduction to spectroscopy and laws of light, Microscopy - Principle and application of phase contrast, Fluorescence and Electron microscopy, Microtomy - types of microtome, material preparation and fixation - Embedding - block making, Steps involved in sectioning, staining and mounting, Cytophotometry, cryostat VIEW CHAPTERS
3 Unit 3 NA Biophysical method for the determination of biopolymer structure, principles, components and application of X- Ray diffraction, UV - Visible, NMR and ESR Spectroscopy, Calorimeter, Spectrophotometer, pH meter, Principle, Design and applications, LASER - principle and application VIEW CHAPTERS
4 Unit 4 NA Tracer techniques- Radioactive isotope - Half life, GM counter, Liquid scintillation counter, Autoradiography, Fractionation techniques - Ultra centrifugation, Density gradient centrifugation VIEW CHAPTERS
5 Unit 5 NA Electrophoresis -SDS - PAGE, Agarose gel, Immunoelectrophoresis, Chromatography - principle, instrumentation and application of column Chromatography, ion exchange chromatography, Thin layer Chromatography and HPLC VIEW CHAPTERS
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