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BDU rDNA Technology Sem-IV
No Units Titles Sub Titles Chapters
1 Unit 1 NA Outline process of genetic engineering and recombinant DNA technology, Isolation of genes, exonuclease & endonuclease, Concept of restriction and modification - Restriction endonucleases, DNA modifying enzymes, Ligases, Different Kinds of Vectors - Plasmids, Phage vectors, Cosmids, Phagemids, Virus vectors, Shuttle vectors and expression vectors- YAC, BAC- S, cerevisiae system as a model VIEW CHAPTERS
2 Unit 2 NA Host-vector system - Cloning vectors for E, coli,, Cloning vectors for Eukaryotesmethods of transformation - Cloning strategies, construction of genomic libraries and cDNA Libraries, Probe construction, recombinant selection and screening, Molecular cloning VIEW CHAPTERS
3 Unit 3 NA Analysis of expression, Analysis of recombinant DNA (Selection methods - antibiotics, expression basis, GUS expression), sequencing (chemical degradation, chain termination and automated sequence), mutagenesis, altered expression and engineering genes, Sitedirected mutagenesis, DNA amplification using polymerase chain reaction (PCR)- key concepts, Analysis of amplified products, Southern blot, Northern blot and Western blot Applications of PCR - Ligase chain reaction, RFLP, RAPD, DNA Finger printing VIEW CHAPTERS
4 Unit 4 NA Application of rDNA Technology in plants- Transgenic plants with reference to virus and pest resistances, herbicide tolerance and stress tolerance (cold, heat and salt), cytoplasmic male sterility, delay of fruit ripening, resistance to fungi and bacteria, Biopharmaceuticals and secondary metabolite production VIEW CHAPTERS
5 Unit 5 NA Application of rDNA Technology in animals- Transgenic animals -pharmaceutical production, insulin production, farm animal protection, Gene therapy - haemopoietic cells, genetically engineered bone marrow cells, skin fibroblasts, hepatocytes, myoblast and genetically modified lymphocytes - Recombinant Technology in the production of vaccines VIEW CHAPTERS
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