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BDU Plant Tissue Culture Sem-V
No Units Titles Sub Titles Chapters
1 Unit 1 NA Basic techniques and tools-Establishment of plant tissue culture lab- equipment, culture vessels, surface sterilization of various explants,pretreatment of explant, subculture and repeated transfer of explants and cultures VIEW CHAPTERS
2 Unit 2 NA Composition of various tissue culture media and their preparation- Establishment of callus, suspension cultures, organogenesis and embryogenesis - Meristem tip culture- Hardening of plants VIEW CHAPTERS
3 Unit 3 NA Techniques of anther, embryo and ovule culture- Protoplast isolation, culture and fusion, Artificial seed (synthetic seed) - Cell line selection using selection pressure- Production of secondary metabolites- Cryopreservation VIEW CHAPTERS
4 Unit 4 NA Isolation and characterisation of nuclei and nucleoli, isolation and functional analysis of mitochondria, chloroplast, Preparation and analysis of genetic material -cell autoradiography, aspetic technique and media preparation of primary cultures, maintenance of secondary culture -cell line propogation -cells in suspension VIEW CHAPTERS
5 Unit 5 NA Analysis of biosynthesis of cellular components by radioactive labeling of cultured cells, Plant cell structure and organisation of cell groups in tissue system, Mass culture of plant cell suspension, somaclones, mericloning, micropropagation VIEW CHAPTERS
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