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MSRIT Bioanalytical Techniques Sem 4
No Units Titles Sub Titles Chapters
1 Unit 1 Introduction Types & Basic concepts of analytical methods, instruments for analysis, electromagnetic radiation - its properties and interaction with matter, Emission of radiation, Preparation of standard solutions and buffers, pH and Oxygen electrodes, An introduction to absorption spectroscopy, photometry, beer lamberts law VIEW CHAPTERS
2 Unit 2 Spectroscopy UV, visible, IR spectrophotometry theory and instrumentation, Turbidometry & Nephelometry, Principles & Applications, Spectrofluorimetry, Principles & Applications, Flame Emission and Atomic Absorption Spectroscopy, Principles & Instrumentation, NMR Spectroscopy, Principles, Instrumentation, Applications Raman Spectroscopy, Principles, Instrumentation, Applications VIEW CHAPTERS
3 Unit 3 Mass Determination Mass Spectrometry, Principles, Instrumentation, and applications, Centrifugation  Principles of centrifugation, concepts of RCF, different types of instruments and rotors, preparative, differential and density gradient centrifugation, analytical ultra-centrifugation, determination of molecular weights and other applications, subcellular fractionation VIEW CHAPTERS
4 Unit 4 Chromatography Distribution coefficient, modes of chromatography like Paper, Thin Layer, Molecular Exclusion chromatography, Principles, Instrumentation, Applications, Ion Exchange and Affinity chromatography  Principles & Applications Gas liquid chromatography, Principles, Instrumentation, Applications, High Pressure Liquid Chromatography (HPLC), Principles, Instrumentation, Applications VIEW CHAPTERS
5 Unit 5 Electrophoresis General principles, Support media, Electrophoresis of proteins  SDS PAGE, Native PAGE, Gradient gel electrophoresis, 2D gel electrophoresis, Isoelectric focusing, Electrophoresis of Nucleic acids- Agarose Gel Electrophoresis VIEW CHAPTERS
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