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MSRIT Molecular Biology Sem 4
No Units Titles Sub Titles Chapters
1 Unit 1 Introduction to Molecular Biology Scope of molecular biology, Genomes, genetic material, gene structure and functions, Concept of central dogma of molecular biology, DNA replication: Principle, General features of DNA replication, DNA replication in prokaryotes, prokaryotic DNA polymerases, mechanism of E, coli DNA replication and other DNA replication models (rolling circle model, unidirectional replication), DNA replication in eukaryotes: eukaryotic, DNA polymerases, mechanism of DNA replication and chromosome-end replications, replication fidelity, DNA damage & Repair, causes, types of mutations, repair mechanisms (direct reversal, excision, and SOS) VIEW CHAPTERS
2 Unit 2 Transcription What is transcription∞, Structure and sequences of prokaryotic and eukaryotic genes, Components of transcription unit, Bacterial and eukaryotic RNA polymerases, transcription factors, mechanism of transcription in prokaryotes and eukaryotes, transcriptional apparatus, Posttranscriptional processing of RNA: 5 and 3 end modifications, splicing (type-I, type II and spliceosome-mediated), alternative splicing and its biological significance, exon shuffling and RNA editing VIEW CHAPTERS
3 Unit 3 Translation Principle, genetic code and codons, Components of translation, Activation of tRNA, Mechanism of translation, initiation, elongation and termination of protein synthesis, Main differences between prokaryotic and eukaryotic translation, Post translational modifications, cleavage, methylation, sulfur-sulfur bridges, protein splicing, folding, glycosylation, myristilation, Protein targeting, signal hypothesis and cotranslational processing, transportation, Inhibitors of transcription and protein synthesis VIEW CHAPTERS
4 Unit 4 Gene Expression Regulation Regulation of gene expression in prokaryotes, Operon model, significance of operons, lac and trp operons, positive versus negative regulation, Role of sigma factors in gene expression regulation, Gene rearrangements in gene expression regulation, Eukaryotic gene expression regulation, Role of upstream activating sequences and regulatory proteins, Transcriptional and translational control of gene expression, mRNA turnover kinetics, Gene silencing: anti-sense RNA, RNAi, Posttranslational regulation, protein degradation and turnover VIEW CHAPTERS
5 Unit 5 Genome Modifications Genetic recombination in bacteria and viruses, site-specific recombination as in meiosis, illegitimate recombination, Recombination repair mechanisms, Role of recombination and transposition in evolution of new genes, Transposons and mechanism of transposition, insertion sequences in bacteria, mobile elements of maize (McClintock s work), Biological significance of transposition VIEW CHAPTERS
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