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MSRIT Genetic Engineering Sem 5
No Units Titles Sub Titles Chapters
1 Unit 1 Introduction Nature of genetic material, structure and functions of genes, co-linearity of genetic code, Gene expression and regulatory sequences, Introduction to recombinant DNA technology, vectors in recombinant DNA technology, biology and salient features of vectors, types of cloning vectors, Bacterial plasmids, Ti, cosmids, and phages, Animal vectors, SV 40, Adenovirus, papiloma virus, Baculovirus, Plant vectors, Ti plasmids, TMV, CaMV and Gemini virus, Artificial chromosomes, BACs, YACs and mini-chromosomes, Cloning host systems, Bacteria, Yeast, animals, Plants, and Cell lines VIEW CHAPTERS
2 Unit 2 Enzymes in Genetic Engineering and Construction of DNA libraries DNApolymerases, Nucleases, exo- and endo-nucleases, Restriction endonucleases, classification, mode of action and applications, Enzymes in modification of DNA, DNA methylases, phosphatases, kinases, Ligases, RNase, topoisomerases, Construction and screening of DNA libraries, genomic and cDNA libraries, Isolation of nucleic acids, plasmids and genomic DNA, Preservation of recombinant molecules VIEW CHAPTERS
3 Unit 3 Nucleic acid Detection and Characterization Methods of nucleic acid detection, cytological detection, polymerase chain reaction (PCR), Detection by hybridization, probe and target sequences, Southern blotting, Northern blotting and in situ hybridization techniques, Nucleic acid mutagenesis, applications in strain improvement and generation of genetic diversity (site-specific mutagenesis), Nucleotide sequencing, Sanger and Automated DNA sequencing VIEW CHAPTERS
4 Unit 4 Gene Transfer Techniques Importance of gene transfer and types (Vector, chemical and physical), Gene transformation of bacteria, natural (transformation, conjugation and transduction) Chemical (Calcium chloride precipitation), Gene transfer techniques in animals by vectors (SV 40 and retroviral) chemical (liposome) and by physical (microinjection, electroporation and gene gun), Gene transfer in plants, vector-mediated (Agrobacterium-mediated gene transfer in plants and its applications), Non-vector mediated transformation, microinjection and macro-injection, liposome, electroporation, Gene-gun VIEW CHAPTERS
5 Unit 5 Application of Recombinant DNA Technology Genetically modified organisms (GMOs), Techniques of gene mapping and utilization, RFLP, RAPD, Marker-assisted selection and breeding for improvement, Transgenic plants, plants as bioreactors, transgenic crops for increased yield, resistance to biotic and abiotic stresses, Transgenic animals-as bioreactor for production of proteins and pharmaceuticals, Genetically modified microbes (GMM), GMMs for production of antibiotics, enzymes, Insulin, growth hormones, monoclonal antibodies, clearing oil spills, Recombinant technology in Human health, Diagnosis and applications of human diseases (molecular probes, immuno-detection, drug targeting of selective cells or tissues), Biosafety regulations and evaluation of genetically modified organisms (GMOs) VIEW CHAPTERS
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