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MSRIT Enzyme Technology Sem 6
No Units Titles Sub Titles Chapters
1 Unit 1 Introduction to enzymology Introduction to enzymes, Advantages of enzymes vs chemical catalysts, Classification, Active site, Mechanism of enzyme action, enzyme specificities, Strategies of purification of enzymes, criteria of purity, molecular weight determination, Introduction to coenzymes & co-factors VIEW CHAPTERS
2 Unit 2 Enzyme kinetics Enzyme substrate reaction, Derivation of Michaelis and Menten equation, Lineweaver Burk plot, Units of enzyme activity, Enzyme inhibition, Competitive, Non-Competitive, Uncompetitive, and Irreversible inhibition, Multisubstrate reactions, ping-pong, Sequential, ordered and random mechanism, Regulation of enzyme activity, Allosteric enzymes, Feedback inhibition, Covalent modification, Proteolytic cleavage VIEW CHAPTERS
3 Unit 3 Enzymatic techniques Enzyme and isoenzyme measurement methods with two examples, Methods for investigating the kinetics of enzyme catalyzed reactions, Initial velocity studies, rapid-reaction techniques, Design and construction of novel enzymes, artificial enzymes, Catalytic antibodies VIEW CHAPTERS
4 Unit 4 Medical applications Enzymes of Biological Importance, Importance of enzymes in diagnosis, Enzyme pattern in diseases like Myocardial infarctions (SGOT, SGPT, & LDH), Isoenzymes (CK, LD, ALP), Use of isozymes as markers in diseases, Enzymes in immunoassay techniques, Therapeutic enzymes VIEW CHAPTERS
5 Unit 5 Industrial applications Techniques of enzyme immobilization, applications of immobilized enzyme technology, Economic argument for immobilization, Enzymes used in detergents, use of proteases in food and leather industries, methods involved in production of glucose syrup from starch (using starch hydrolyzing enzymes), production of glucose from cellulose and sucrose, uses of lactase in dairy industry, glucose oxidase and catalase in food industry VIEW CHAPTERS
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