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MSRIT Upstream Process Technology Sem 6
No Units Titles Sub Titles Chapters
1 Unit 1 Introduction to plant tissue culture techniques Media constituents & selection concept of cellular totipotency and its applications, Basic plant tissue culture techniques, Callus induction, somatic embryogenesis (SE), large scale production of SE s, production of Artifical seeds, Bioprocess consideration in using plant cell cultures, Bioreactors for suspension cultures, immobilized cells and for organized tissues, important Phytopharmaceuticals (ergolins, vinca alkaloids, shikonin) VIEW CHAPTERS
2 Unit 2 Industrially important metabolites Production and recovery of industrially important primary and secondary metabolites, glutamic acid, Vit B12, ethanol, antibiotics (penicillin, streptomycin, erythromycin), microbial polysaccharides VIEW CHAPTERS
3 Unit 3 Animal cell culture techniques Media for culturing cells and tissues, natural and defined media, Preparation of various tissue culture media, sterilization and storage, Sterilization of various equipments and apparatus Short-term lymphocyte culture (suspension cultures) Fibroblast cultures from chick embryo, Hybridoma and monoclonal antibody production, In vitro culture of oocytes/embryos, Cell/embryo cryopreservation, Stem cell isolation and culture, Bioreactors considerations for animal cell cultures, Production of Monoclonal antibodies VIEW CHAPTERS
4 Unit 4 Microbial cell culture techniques Sterilization, Media preparation, Culture maintenance, Single colony purification, Bacterial titer estimation, Growth curve, Replica plating, Culture characterization, Auxotroph isolation of Viruses and bacteriophages, Biochemical characterization, Antibiotic sensitivity Strain improvement, Conjugational genetic transformation, Generalized transduction VIEW CHAPTERS
5 Unit 5 Fermentation technology Medium formulation, design operation of a typical aseptic, aerobic fermentation process, alternate bioreactor configuration, Optimization of fermentations, Germplasm Storage technique, microbial leaching, Improvement of Existing Antibiotic Classes VIEW CHAPTERS
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