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MSRIT Genomics and Proteomics Sem 6
No Units Titles Sub Titles Chapters
1 Unit 1 DNA & Genome Sequencing DNA sequencing methods, Sanger dideoxy method, Maxam Gilbert method, Thermal cycling method, Pyrosequencing, Automated Fluorescence method, DNA-chip method, introduction to Next Generation Sequencing technology (NGS), Methods of preparing genomic DNA for sequencing, BAC & YAC vectors, Shotgun approaches, Clone contig approach VIEW CHAPTERS
2 Unit 2 Genome projects & Genome analysis Genome size and C-value paradox, Genome sequencing projects of E, coli, Yeast, Drosophila, Rice & Humans, Organelle genomes, Chloroplast, Mitochondria, Importance of noncoding sequences- microRNA, ShRNA, PiWi, RNA interference, Finding genes in genomes, principle, of gene prediction / gene modeling VIEW CHAPTERS
3 Unit 3 Functional & Comparative Genomics Constant volume batch reactor, first order, second order, zero order reactions, fractional life, homogenous catalyzed reactions, irreversible reaction in series, autocatalytic reactions, reversible reactions, integral and differential method of analysis VIEW CHAPTERS
4 Unit 4 Proteomics Methods of protein isolation, purification, quantification, Analysis of proteins, high throughput screening, Yeast-two-hybrid interaction screens, 2D electrophoresis, Mass spectrometry, Peptide fingerprinting, MALDI-TOF, protein  protein interaction maps, post-translational modification analysis, protein chips, proteomics databases & analysis, Metabolomics, Metabolic pathway databases, KEGG VIEW CHAPTERS
5 Unit 5 Structural Genomics Structural genomics, Introduction, Structural genomics initiative, high throughput structure determination, Principle, salient features & drawbacks of methods of prediction of protein secondary structure, Chou-Fasman, GOR, PSI-PRED, PROF, PHD, Principle, salient features of methods of prediction of tertiary structure of proteins, Comparative protein modeling/Homology modeling, threading & Ab initio structure prediction VIEW CHAPTERS
 
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