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MSRIT Animal Biotechnology Sem 6 Elective
No Units Titles Sub Titles Chapters
1 Unit 1 Introduction to animal biotechnology Introduction, History and Scope, Cell culture Laboratory design & Equipments, Layout, Maintenance of sterility, CO2 incubator, Inverted stage microscope, Cell culture vessels, Cryopreservation, Media and reagents, CO2 and bicarbonates buffering VIEW CHAPTERS
2 Unit 2 Animal cell culture Different tissue culture techniques, primary culture, Secondary culture, Trypsinization, Continuous cell lines, Suspension culture, Organ culture etc, Behavior of cells in culture conditions, Morphology, division, growth pattern, Development of cell lines (Embryonic & Adult), Characterization and maintenance of cell lines, stem cells VIEW CHAPTERS
3 Unit 3 Animal cell culture applications Cell cloning and selection, Transfection and its methods, Commercial scale production of animal cells, stem cells and their application, Application of animal cell culture for in vitro testing of drugs, Application of cell culture technology in production of human and animal viral vaccines VIEW CHAPTERS
4 Unit 4 Development and use of transgenic animals Transgenic animals, Transgenic-mice methodology, Retroviral vector method, DNA microinjection method, Engineeredembryonic stem cell method, Knocking in and knocking out of genes, Applications, transgenic animals as bioreactors for production of proteins of pharmaceutical value, Ethical issues VIEW CHAPTERS
5 Unit 5 Biotechnology for animal improvement Conventional methods of animal improvement, cross breeding, artificial insemination, in vitro fertilization, embryo transfer technology, transgenic animals & their applications, Gene mapping, marker assisted selection and genetic improvement of desired characters of domestic animals, Rapid diagnosis of diseases in live-stock via, RIA, ELISA and PCR VIEW CHAPTERS
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